학술논문
Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.
Document Type
Article
Author
Source
Subject
*EMBRYOS
*BACTERIAL RNA
*BACTERIAL genomes
*MESSENGER RNA
*ENDONUCLEASES
*EUKARYOTES
*MICROINJECTION (Cytology)
*LABORATORY mice
*GENETIC mutation
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Language
ISSN
1548-7091
Abstract
Bacterial RNA-directed Cas9 endonuclease is a versatile tool for site-specific genome modification in eukaryotes. Co-microinjection of mouse embryos with Cas9 mRNA and single guide RNAs induces on-target and off-target mutations that are transmissible to offspring. However, Cas9 nickase can be used to efficiently mutate genes without detectable damage at known off-target sites. This method is applicable for genome editing of any model organism and minimizes confounding problems of off-target mutations. [ABSTRACT FROM AUTHOR]