부산대학교 도서관

Objectives This study has intended to investigate longevity of subcutaneous fat-derived mesenchymal stem cells ( SF- MSCs) under extensive culturing. It has also focused on optimization of culture media for them over prolonged periods in vitro. Materials and methods We evaluated SF- MSCs with reference to phenotypic characterization, proliferative ability, karyotype stability and differentiation potency with early (P3) and late passage (P20) conditions, using four different media, DMEM- LG, ALPHA- MEM, DMEM-F12 and DMEM- KO. Results This study unravels retention of SF- MSC characteristics in facets of phenotypic expression profile ( CD 90, CD 105, CD 73, CD 34, CD 29, CD 54, CD 49d, CD 117, HLA- DR, CD 166, CD 31, CD 44), proliferative characteristics, karyotyping and differentiation potency prolonged culturing to P25 in all media. Population doubling time ( PDT) in Alpha MEM, DMEM LG, DMEM F 12, DMEM KO were identified to be (1.81, 1.84, 1.9, 2.08 days) at early passage and (2.93, 2.94, 3.12, 3.06 days) at late passage. As a corollary, Alpha MEM and DMEM LG serve as appropriate basal media for SF- MSC when proliferative potency is considered. Conclusions In research, it is imperative that SF- MSC uphold their expansion potency in the aforesaid attributes in all media over extensive culturing, thereby transforming their colossal in vitro potency, with the aim of curing a wide horizon of diseases. [ABSTRACT FROM AUTHOR]