학술논문
ATP-dependent Ca2+ transport in vesicles isolated from the bile canalicular region of the hepatocyte plasma membrane.
Document Type
Article
Source
Subject
*CELL membranes
*ADENOSINE triphosphate
*LIVER cells
*CELLS
*BLOOD
*
*
*
*
Language
ISSN
0014-2956
Abstract
Three plasma membrane subfractions have been isolated and characterized from rat liver cells. The high affinity Ca2+-stimulated ATPase is highly enriched the bile canalicular subfraction. Taking into account cross- contamination by the blood sinusoidal and lateral membranes it is suggested that the high-affinity Ca2+-ATPase is located exclusively in this fraction. The high-affinity Ca2+-ATPase is coupled to Ca2+ transport, is calmodulin- insensitive, sensitive to vanadate under appropriate experimental conditions and is strongly inhibited by La3+. In the presence of Ca2+ and ATP the ATPase forms a phosphorylated intermediate of molecular mass about 200 kDa. [ABSTRACT FROM AUTHOR]