학술논문
Triplex Direct Quantitative Polymerase Chain Reaction for the Identification of Streptococcus pneumoniae Serotypes.
Document Type
Journal Article
Author
Source
Subject
*STREPTOCOCCUS pneumoniae
*POLYMERASE chain reaction
*SEROTYPES
*CEREBROSPINAL fluid
*STREPTOCOCCAL disease diagnosis
*SEROTYPING
*RESEARCH
*RESEARCH methodology
*EVALUATION research
*STREPTOCOCCUS
*COMPARATIVE studies
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Language
ISSN
0022-1899
Abstract
The quantitative polymerase chain reaction (qPCR) method presented in this study allows the identification of pneumococcal capsular serotypes in cerebrospinal fluid without first performing DNA extraction. This testing approach, which saves time and resources, demonstrated similar sensitivity and a high level of agreement between cycle threshold values when it was compared side-by-side with the standard qPCR method with extracted DNA. [ABSTRACT FROM AUTHOR]