부산대학교 도서관

In the present study, the effect of ingested viable Lactobacillus casei Shirota strain YIT9029 on oral infection with the enteric pathogen Listeria monocytogenes in Wistar rats was investigated. Rats were orally infected with 109 viable L. monocytogenes. Starting 3 days before the infection, rats received a daily dosage of 109 viable L. casei. It was shown that supplementation of L. casei significantly reduced the numbers of L. monocytogenes in stomach, caecum, faeces, spleen and liver, 2 days after L. monocytogenes infection. The number of L. monocytogenes in the mesenteric lymph nodes was not affected by the ingestion of L. casei. In comparison with control animals, the levels of the liver-specific alanine aminotransferase were lower in L. casei-fed rats. Histological analysis of spleen and liver revealed no differences between the experimental and control animals. In a parallel study with orally L. monocytogenes infected rats, it was shown that L. casei was able to increase cellular immunity significantly as determined with the delayed-type hypersensitivity response against heat-killed L. monocytogenes. In conclusion, in the present study it was shown that orally administered L. casei is able to enhance host resistance against oral L. monocytogenes infection. In the gastrointestinal tract, as well as in the spleen and liver, L. monocytogenes numbers were reduced. Furthermore, it is concluded that the enhancement of this anti-Listeria activity might be, at least partly, due to increased cell-mediated immunity. [Copyright &y& Elsevier]