부산대학교 도서관

The mechanisms controlling wiring of neuronal networks are not completely understood. The stereotypic architecture of the Drosophila mushroom body (MB) offers a unique system to study circuit assembly. The adult medial MB γ‐lobe is comprised of a long bundle of axons that wire with specific modulatory and output neurons in a tiled manner, defining five distinct zones. We found that the immunoglobulin superfamily protein Dpr12 is cell‐autonomously required in γ‐neurons for their developmental regrowth into the distal γ4/5 zones, where both Dpr12 and its interacting protein, DIP‐δ, are enriched. DIP‐δ functions in a subset of dopaminergic neurons that wire with γ‐neurons within the γ4/5 zone. During metamorphosis, these dopaminergic projections arrive to the γ4/5 zone prior to γ‐axons, suggesting that γ‐axons extend through a prepatterned region. Thus, Dpr12/DIP‐δ transneuronal interaction is required for γ4/5 zone formation. Our study sheds light onto molecular and cellular mechanisms underlying circuit formation within subcellular resolution. Synopsis: Precise circuit formation is crucial for neuronal function. Here, we identify trans‐synaptic interactions of Immunoglobulin Superfamily proteins as mediators of compartmentalized dopaminergic innervation in the Drosophila mushroom body. The IgSF protein Dpr12 is cell‐autonomously required in γ‐Kenyon cells for their complete developmental regrowth.Dpr12 and its interacting protein DIP‐δ are both enriched in the MB γ4–5 zones, defined by compartmentalized innervations of dopaminergic and MB output neurons.DIP‐δ functions within a cluster of dopaminergic neurons that innervates the γ4–5 zones.The Dpr12‐DIP‐δ interaction is required for the formation of the γ4–5 zones.DIP‐δ is required and sufficient for the localization of Dpr12, in trans. [ABSTRACT FROM AUTHOR]