학술논문
Luteolin Overcomes Resistance to Benzyl Isothiocyanate- Induced Apoptosis in Human Colorectal Cancer HCT-116 Cells.
Document Type
Article
Author
Source
Subject
*ANALYSIS of variance
*APOPTOSIS
*BIOLOGICAL assay
*CELL culture
*COLON tumors
*GENE expression
*ONCOGENES
*POLYMERASE chain reaction
*RESEARCH funding
*T-test (Statistics)
*PHYTOCHEMICALS
*DATA analysis software
*FLAVONES
*DESCRIPTIVE statistics
RECTUM tumors
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Language
ISSN
1021-9498
Abstract
We have previously identified p53, a universal sensor of genotoxic stress, as a negative regulator of the apoptosis induction by benzyl isothiocyanate (BITC) in the normal colon fibroblastoid cells. In the present study, we further confirmed that BITC has a potential to induce cytotoxicity in the p53-mutated colon cancer HT-29 cells in preference to HCT-116 cells with wild-type p53. To obtain effective induction of BITC-stimulated apoptosis in p53-positive cells, we investigated the combination effect of BITC and food ingredient that may overcome resistance to BITC. Pretreatment with luteolin potentiated the cytotoxicity induction by BITC in HCT-116 cells but not in HT-29 cells. The biochemical events related to apoptosis such as DNA ladder formation and caspase-3 activation were also enhanced by luteolin. Luteolin attenuated the expression of p21wafl/cipl, a key downstream target of p53. These results suggest the role of p21wafl/cipl pathway in the overcoming BITC resistance by luteolin. [ABSTRACT FROM AUTHOR]