학술논문
RESEARCH NOTE The reproducibility of an enzyme-linked immunosorbent assay for detection of Chlamydia pneumoniae-specific antibodies.
Document Type
Other
Author
Source
Subject
*ENZYME-linked immunosorbent assay
*CHLAMYDOPHILA pneumoniae
*IMMUNOGLOBULINS
*VASCULAR diseases
*CEREBROVASCULAR disease patients
*SERUM
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Language
ISSN
1198-743X
Abstract
With use of an enzyme-linked immunosorbent assay (ELISA), Chlamydia pneumoniae immunoglobulins were detected in a consecutive series of patients' sera ( n = 122 for IgA and IgG; n = 138 for IgM). When the ELISA tests were repeated, the percentage disagreements were 12%, 16% and 10% for C. pneumoniae IgA, IgG and IgM, respectively. The reproducibility of the ELISA, expressed as kappa values, for IgA, IgG and IgM was 0.73, 0.60 and 0.53, respectively (p < 0.001). It was concluded that the ELISA had good reproducibility for detecting C. pneumoniae IgA, and moderately good reproducibility for detecting C. pneumoniae IgG and IgM. [ABSTRACT FROM AUTHOR]